epidermoid carcinoma cell line  (ATCC)

Journal: The Journal of Biological Chemistry

Article Title: Hyaluronic acid regulates cellular UDP-GlcNAc levels through CD44 to affect glycosylation and cell biological functions

doi: 10.1016/j.jbc.2025.111111

Figure Lengend Snippet: Establishment of HAS2 KO cells and confirmation of HA synthesis. A , comparison of HAS2 expression levels among different cell lines: HeLa (cervical adenocarcinoma cell line), A431 (epidermoid carcinoma cell line), PANC-1 (pancreatic ductal adenocarcinoma cell line), MIA PaCa-2 (pancreatic carcinoma cell line), and HepG2 (hepatocellular carcinoma cell line). The HAS2 mRNA level in HeLa was normalized to 1.0. All values are presented as mean ± SD from three independent experiments, based on one-way ANOVA with Tukey’s post hoc analysis. ∗∗∗ p < 0.001. B , comparison of HAS1 , HAS2 , and HAS3 expression levels in HeLa cells. The HAS1 mRNA level in HeLa was normalized to 1.0. All values are presented as mean ± SD from three independent experiments, based on one-way ANOVA with Tukey’s post hoc analysis. ∗∗∗ p < 0.001. C , Sanger sequencing was used to analyze exon 2 of the target sequence in HeLa WT and HAS2 KO cells with the canonical SpCas9 PAM sequence (TGG) highlighted in bold. D , WT and HAS2 KO cells were cultured in serum-free medium, and the conditioned medium was collected after 24 h to measure HA concentration. Statistical significance was determined from three independent experiments using an unpaired Student’s t test, with p values indicated as ∗∗∗ p < 0.001.

Article Snippet: A431 cells were obtained from ATCC.

Techniques: Comparison, Expressing, Sequencing, Cell Culture, Concentration Assay

Journal: bioRxiv

Article Title: IL-1α drives a tumor-stroma-neutrophil axis through inflammatory fibroblast activation in head and neck cancer

doi: 10.64898/2026.01.20.700440

Figure Lengend Snippet: (A) MSCs were stimulated for 24 h with FaDu tumor-conditioned SNs. Tumor-derived cytokines (columns) were quantified by Luminex, and MSC-derived CXCL8 and G-CSF (rows) by ELISA. Data are displayed as a correlation matrix. (B) MSCs were treated with FaDu tumor SNs for 24 h. Tumor-derived factors were analyzed by Luminex; MSC-derived CXCL8 and G-CSF were quantified by ELISA. Tumor-derived IL-1α correlates with MSC-derived CXCL8 and G-CSF. (C) MSCs were treated with recombinant IL-1α for 24 h. Release of CXCL8 and G-CSF was analyzed by ELISA. (D) IL-1α was measured in control (non-sense, NS) and IL-1α overexpressing FaDu cells (IL-1α-OE) using Luminex. (E) MSCs were treated with SNs from non-sense and IL-1α-OE cells. MSC-derived CXCL8 and G-CSF were quantified by ELISA. (F) IL-1α release was determined in the SN of viable and necrotic FaDu and A431 cells. MSCs were treated with the SN of viable and necrotic FaDu (G) or A431 (H) cells for 24 h. MSC-derived CXCL8 and G-CSF were quantified by ELISA. Statistical significance was assessed after log-transformation using an ordinary one-way ANOVA with Tukey’s multiple comparisons test (C), while paired t -tests were applied for panels D-H. Data are shown as mean ± SD. In panel C, significance levels are indicated as # or * (p ≤ 0.05), ## or ** (p ≤ 0.01), ### or *** (p ≤ 0.001), and #### or **** (p ≤ 0.0001); all other p values are shown numerically. Symbols (BioRender) are included to show the origin of the analyzed SNs.

Article Snippet: Human carcinoma cell lines: The epidermoid carcinoma cell line A431 (ATCC CRL-1555) was cultured in DMEM with 4.5 g/L glucose (Pan-Biotech, Aidenbach, Germany), supplemented with 10% heat inactivated FCS (BioSell, Feucht, Germany) and 100 units/ml penicillin and 100 μg/mL streptomycin (Thermo Fisher Scientific, Waltham, USA).

Techniques: Derivative Assay, Luminex, Enzyme-linked Immunosorbent Assay, Recombinant, Control, Transformation Assay

Journal: bioRxiv

Article Title: IL-1α drives a tumor-stroma-neutrophil axis through inflammatory fibroblast activation in head and neck cancer

doi: 10.64898/2026.01.20.700440

Figure Lengend Snippet: (A,B) Tumor-derived cytokines were quantified by Luminex analysis. (A) Data are displayed as Pearsońs correlation matrix. Significant correlations among tumor-derived factors are indicated by black asterisks (B). Quantification of tumor-derived mediators known to be involved in tumor-stroma communication. (C) MSCs were treated with FaDu-CXCL8KO tumor conditioned medium in the presence of 10 µg/mL IL-1α neutralizing antibody or isotype control. Released CXCL8 and G-CSF were quantified by ELISA. (D) Quantification of tumor-derived mediators of tumor-stroma communication in non-sense and IL-1α overexpressing (IL-1α-OE) cells was performed by Luminex. (E) Quantification of tumor-derived factors implicated in neutrophil recruitment and survival in SN from non-sense and IL-1α-OE cells (Luminex and CXCL8-ELISA). (F) LDH assay quantifying cytotoxicity in SN of viable and necrotic FaDu and A431 tumor cells. Statistical analysis was performed using paired t -tests (C,F) and unpaired t -tests (D). Data are presented as mean ± SEM (C) and mean ± SD (D,E), p values are indicated.

Article Snippet: Human carcinoma cell lines: The epidermoid carcinoma cell line A431 (ATCC CRL-1555) was cultured in DMEM with 4.5 g/L glucose (Pan-Biotech, Aidenbach, Germany), supplemented with 10% heat inactivated FCS (BioSell, Feucht, Germany) and 100 units/ml penicillin and 100 μg/mL streptomycin (Thermo Fisher Scientific, Waltham, USA).

Techniques: Derivative Assay, Luminex, Control, Enzyme-linked Immunosorbent Assay, Lactate Dehydrogenase Assay